Ascorbate oxidase based amperometric biosensor for organophosphorous pesticide monitoring.

Rekha, K and Gouda, M. D. and Thakur, M. S. and Karanth, N. G. (2000) Ascorbate oxidase based amperometric biosensor for organophosphorous pesticide monitoring. Biosensors & bioelectronics, 15 (9-10). pp. 499-502. ISSN 0956-5663

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Abstract

An amperometric principle based biosensor containing tissues of cucumber, rich in ascorbic acid oxidase, was used for the detection of organophosphorous (OP) pesticide ethyl paraoxon, which inhibits the activity of ascorbic acid oxidase. The optimal concentration of ascorbic acid used as substrate was found to be 5.67 mM. The biosensor response was found to reach steady state within 2 min. A measurable inhibition (> 10%) was obtained with 10 min incubation of the enzyme electrode with different concentrations of the pesticide. There was a linear relationship between the percentage of inhibition of the enzyme substrate reaction and the pesticide (ethyl paraoxon) concentration in the range 1-10 ppm with a regression value 0.9942.

Item Type:	Article
Uncontrolled Keywords:	Ascorbate oxidase; Organophosphorous pesticide; Amperometry; Biosensor
Subjects:	600 Technology > 03 Agriculture
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Aug 2008 10:08
Last Modified:	18 Nov 2016 10:33
URI:	http://ir.cftri.res.in/id/eprint/2131

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