Non-cellulosic mixed linkage beta-D-glucan in sorghum, Sorghum bicolor (L.) Moench--localization and biological activity studies.

Ramesh, H. P. and Tharanathan, R. N. (2000) Non-cellulosic mixed linkage beta-D-glucan in sorghum, Sorghum bicolor (L.) Moench--localization and biological activity studies. Indian Journal of Experimental Biology, 38 (2). pp. 155-159. ISSN 0019-5189

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Abstract

Fluorescence of Calcofluor and Congo Red was observed in stained sections of sorghum grain (Sorghum bicolor (L.) Moench), especially in the sub-aleurone cells indicating the presence of mixed linkage beta-D-glucan. Relatively less fluorescence intensity was observed in the single layered (approximately 20 microns thick) aleurone. Alkali extracted beta-D-glucan (fraction 2) of sorghum showed 30% activation of rat peritoneal macrophages (in vitro) at 100 micrograms ml-1 concentration in 10 min. This activation was found mediated mainly through PLA2 pathway. A phagocytic index k of 0.102 +/- 0.008 was observed in vivo carbon clearance test in mice in the group treated with fraction 2. Accumulation of colloidal carbon particles in spleen and liver of mice was moderate in this group, compared to control.

Item Type:	Article
Uncontrolled Keywords:	sorghum, beta-d-glucans, congo red, calcofluor dyes
Subjects:	600 Technology > 08 Food technology > 21 Cereals > 06 Sorghum 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Jul 2011 04:28
Last Modified:	22 Jul 2011 04:28
URI:	http://ir.cftri.res.in/id/eprint/2138

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