Computer-aided modeling and optimization for capsaicinoid production by immobilized Capsicum frutescens cells.

Suvarnalatha, G. and Chand, N. and Ravishankar, G. A. and Venkataraman, L. V. (1993) Computer-aided modeling and optimization for capsaicinoid production by immobilized Capsicum frutescens cells. Enzyme and Microbial Technology, 15 (8). pp. 710-715. ISSN 0141-0229

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Abstract

Production of capsaicinoids by immobilized cell cultures of Capsicum entrapped in calcium alginate matrix is influenced by several factors related to immobilization techniques, including media composition and the biosynthetic capability of cells. Central composite rotatable design (CCRD), one of the response surface methodologies, was used to arrive at optimal conditions for maximizing the yields of capsaicinoids in only 53 experiments, instead of 15,625. Immobilization of cells in 2.5% sodium alginate with 15 min contact time in 60 mM calcium chloride and culture in Murashige and Skoog medium supplemented with 3 mg l-1 2,4-dichlorophenoxyacetic acid and 0.2% curdlan gave the highest yield of 220 micrograms g-1 in 3 days.

Item Type:	Article
Uncontrolled Keywords:	Capsicum', growth regulators: elicitors: immobilization: response surface methodology, optimization: capsaicinoids
Subjects:	600 Technology > 08 Food technology > 31 Food Additives 600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions:	Plant Cell Biotechnology Sensory Science
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Jun 2011 11:10
Last Modified:	06 Jul 2015 08:21
URI:	http://ir.cftri.res.in/id/eprint/2286

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