Gas concentration and temperature gradients in a packed bed solid-state fermentor.

Gowthaman, M. K. and Raghavarao, K.S.M.S. and Ghildyal, N. P. and Karanth, N. G. (1993) Gas concentration and temperature gradients in a packed bed solid-state fermentor. Biotechnology Advances, 11 (3). pp. 611-20. ISSN 0734-9750

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Abstract

In solid-state fermentation (SSF), interaction of heat and mass transfer with biochemical reaction (growth associated enzyme production) affects the bioreactor performance. This interaction was earlier observed to cause temperature and gaseous concentration gradients which reduced the effective bed height of the bioreactor. Since forced aeration is known to alleviate this problem, a packed column bioreactor with forced aeration was employed in the present study. Using wheat bran and Aspergillus niger CFTRI 1105, experiments were conducted for the production of the enzyme amyloglucosidase at various air flow rates. Temperatures and gas concentrations were recorded and enzyme activities estimated at different bed heights during the course of SSF. Gas concentration and temperature gradients decreased with increasing air flow rate. The packed column allowed the use of larger bed heights and yielded higher enzyme activities (6,260 Units/gDMB) than trays (345 Units/gDMB). Enzyme activity was affected more by temperature than concentration gradients, and increased with air flow rates.

Item Type:	Article
Uncontrolled Keywords:	Packed column, forced aeration, temperature, gas concentration, gradients, enzyme activity
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	02 Jun 2011 06:57
Last Modified:	04 Jun 2012 10:05
URI:	http://ir.cftri.res.in/id/eprint/2295

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