Determination of Major Carotenoids in a Few Indian Leafy Vegetables by High-Performance Liquid Chromatography

Lakshminarayana, Rangaswamy and Raju, Marisiddaiah and Krishnakantha, Thirumalai Parthasarathy and Baskaran, V. (2005) Determination of Major Carotenoids in a Few Indian Leafy Vegetables by High-Performance Liquid Chromatography. Journal of Agricultural and Food Chemistry, 53 (8). pp. 2838-2842.

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Abstract

<p align="justify">Leafy vegetables [ Basella rubra L., Peucedanum sowa Roxb., Moringa oleifera Lam., Trigonella foenum-graecumL., Spinacia oleraceaL., Sesbania grandiflora(L.) Poir., and Raphanus sativusL.] that are commonly used by the rural population in India were evaluated in terms of their main carotenoid pattern. The extracted carotenoids were purified by open column chromatography (OCC) on a neutral alumina column to verify their identity by their characteristic UV-visible absorption spectra. Reverse-phase high-performance liquid chromatography (HPLC) on a C18 column with UV-visible photodiode array detection under isocratic conditions was used for quantification of isolated carotenoids. Acetonitrile/methanol/dichloromethane (60:20:20 v/v/v) containing 0.1% ammonium acetate was used as a mobile phase. The major carotenoids identified by both methods were lutein,-carotene, violaxanthin, neoxanthin, and zeaxanthin. Among the carotenoids identified, lutein and ‚-carotene levels were found to be higher in these leafy vegetables. Results show that P. sowa and S. oleraceaare rich sources of lutein (77-92 mg/100 g of dry wt) and ‚-carotene (36-44 mg/100 g of dry wt) compared with other leafy vegetables. The purity of carotenoids eluted by OCC was clarified by HPLC, and they were found to be 92% ( 3% for neoxanthin, 94% ( 2% for violaxanthin, 97% ( 2% for lutein and zeaxanthin, and 90% ( 3% for ‚-carotene. It could be recommended to use P. sowa and S. oleraceaas rich sources of lutein and ‚-carotene for health benefits. The OCC method proposed is relatively simple and provides purified carotenoids for feeding trials.</p>

Item Type:	Article
Uncontrolled Keywords:	Leafy vegetables carotenoids chromatography
Subjects:	600 Technology > 08 Food technology > 23 Vegetables 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Dec 2005
Last Modified:	28 Dec 2011 09:25
URI:	http://ir.cftri.res.in/id/eprint/257

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