Evidence of neuropeptide bivalency in the interaction of substance p with cerabroside sulphate.

Appu Rao, A. G. and Cann, J. R. and Stewart, J. M. and Winzor, D. J. (1990) Evidence of neuropeptide bivalency in the interaction of substance p with cerabroside sulphate. International Journal of Peptide and Protein Research, 35 (3). pp. 250-262.

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Abstract

A precipitin effect has been observed with mixtures of cerebroside sulfate and the neuropeptide substance P. This phenomenon is attributed to multivalency of the lipid due to its existence in micellar form, and to bivalency of substance P. One of those neuropeptide sites is almost certainly the basic residue(s) located at the N-terminal of substance P, whereas the hydrophobic residues at the C-terminus are suggested as candidates for the other site on the basis of turbidimetric, circular dichroic, and fluorometric studies. An intrinsic association constant of 3.6 x 10' M-' has been obtained from the cerebroside sulfate concentration associated with maximal turbidity of mixtures containing a fixed concentration of the neuropeptide.

Item Type:	Article
Uncontrolled Keywords:	cerebroside sulfate; neuropeptide bivalency: substance P; turbidimetry
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	13 Mar 2018 06:02
Last Modified:	27 Oct 2018 10:27
URI:	http://ir.cftri.res.in/id/eprint/4160

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