Influence of medium constituents on growth and pyrethrins production in callus tissues of pyrethrum (Chrysanthemum cinerariaefolium Vis.).

Rajasekaran, T. and Rajendran, L. and Ravishankar, G. A. and Venkataraman, L. V. (1990) Influence of medium constituents on growth and pyrethrins production in callus tissues of pyrethrum (Chrysanthemum cinerariaefolium Vis.). Pyrethrum Post, 17 (4). pp. 121-124.

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Abstract

Callus cultures were initiated from leaf explants of C. cinerariaefolium [Tanacetum cinerariifolium] on MS medium. The cultures were transferred to MS, White's, B-5 or Schenk and Hildebrandt media with 3% sucrose, 2 mg 2,4-D and 5 mg kinetin/litre. Seven C:N:P ratios were compared with a 1:1:1 control (based on 3% sucrose, 840 mg N as ammonium nitrate + potassium nitrate and 170 KH2PO4/litre). Various combinations of IAA, kinetin, IBA, NAA and 2,4-D were also compared. MS medium proved to be most suitable for growth and production of pyrethrins. Callus growth was increased 11-fold with 6% sucrose and 340 mg KH2PO4/litre. The best growth substance combination for production of pyrethrins was 2.0 mg 2,4-D and 5.0 mg kinetin/litre.

Item Type:	Article
Uncontrolled Keywords:	Biotechnology-; in-vitro-culture; metabolites-; production-; Pyrethrins-; Growth-regulators; 2,4-D; kinetin-; tissue-culture; IAA-; NAA-; IBA-; pesticidal-plants; plant-growth-regulators; cytokinins-
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	17 Feb 2022 05:59
Last Modified:	17 Feb 2022 05:59
URI:	http://ir.cftri.res.in/id/eprint/4430

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