Interaction of amino acids, N-acetylamino acid esters, thymine and adenine with sephadex LH-20 GEL.

Prakash, V. and Nandi, P. K. (1975) Interaction of amino acids, N-acetylamino acid esters, thymine and adenine with sephadex LH-20 GEL. Journal of Chromatography, 106 (1). pp. 23-31.

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Abstract

N-Acctyl aromatic amino acid esters, tryptophan, adenine and thymine show strong retention in Sephadex LH-20 gel in an aqueous phase. The decreased retention in 6 M urea. the absence of retention in absolute methanol and the increased retention at higher temperatures in the aqueous phase indicate that hydrophobic interaction is responsible for the observed retention of the amino acids and their esters in the gel. Adenine was found to be retained by polar interaction in the gel. The increased retention of the solutes in the presence of different electrolytes suggests that the lyotropic effect is more important than the ionic strength effect. The relevance of the results obtained with amino acids and esters to the conformational aspects of proteins in aqueous solution is discussed.

Item Type:	Article
Uncontrolled Keywords:	Sephadex LH-20 gel, amino acid esters,
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 03 Amino Acid Chemistry
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	04 Mar 2011 11:36
Last Modified:	07 May 2012 05:35
URI:	http://ir.cftri.res.in/id/eprint/4492

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