In vitro screening on Cytoprotective activity of carotenoids isolated from Dunaliella bardawil -a green alga

Smitha Grace, S. R (2006) In vitro screening on Cytoprotective activity of carotenoids isolated from Dunaliella bardawil -a green alga. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	A microalgae, Dunaliella and its metabolite β-carotene, lutein have been reported to exhibit antioxidant property. It is also known to be possessing anti-inflammatory and anticancer properties. The reaction of carotenoids with radicals is partly due to the roles of carotenoids in photosynthesis, thus electron transfer from b-carotene to P680 ±, with the β-carotene being oxidized to its radical cation carotenoid ± (Telfer et al., 1994). It has been accepted from epidemiological evidence, that dietary b-carotene, mediated by the prevention of lipid peroxidation, can reduce the incidence of many diseases including cancer, atherosclerosis,age-related macular degeneration, and multiple sclerosis (Mares-Perlman et al., 1995; Giovannucci and Clinton, 1998). There have been considerable recent investigations in the interaction of b-carotene and other antioxidants (Mortensen et al., 2001). The antioxidant effects against lead toxicity by Spirulina fusiformis, a blue_/green algae containing rich b carotene and SOD enzyme, were observed on the testes of Swiss mice. Once lead was administered and ROS generated in mice testes, the Spirulina provided antioxidant nutrients of b-carotene and SOD to scavenge the free radicals (Shastri et al., 1999). The interaction of carotenoids and carotenoid radicals with other antioxidants is of importance with respect to anti- and possibly pro-oxidative reactions of carotenoids. The nature of the reaction between the tocopherol (TOH) and various carotenoids shows a marked variation depending on the specific tocopherol homologue, of which a-TOH is the most active. For most carotenoids, including b-carotene, the following reaction was proposed to occur (Mortensen and Skibsted, 1997): In addition, because β-carotene radical cation could interact with vitamin C existing in the aqueous phase, carotenoid radical cations are efficiently reconverted to the parent carotenoid by vitamin C (Mortensen et al., 2001).The management of heavy metal exposure in general population, at large, is being done by the use of chelating agents. The drugs are non-specific, cause trace element imbalance and redistribution of heavy metals among the soft tissues. To overcome/minimize such side effects of conventional chelating agents, the use of commercially available super food Dunaliella appears to be a great choice. There is no report available in literature regarding metal detoxification on neither on animal system as well as on in vitro cell culture system by algal super food such as Dunaliella. Thus, intake of antioxidants originating from Dunaliella is very likely to help in the abatement of the severe manifestations of heavy metal exposure. The immense nutritional value of Dunaliella and their metabolite ready acceptance as dietary supplement by general population make this micro alga as ideal candidate, in the management of heavy metal toxicity.
Uncontrolled Keywords:	Cytoprotective activity Dunaliella bardawil green alga carotenoids
Subjects:	600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Mar 2007
Last Modified:	28 Dec 2011 09:26
URI:	http://ir.cftri.res.in/id/eprint/462

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