Isolation and characterization of the major protein fraction of sunflower seeds.

Rahma, E. H. and Narasinga Rao, M. S. (1981) Isolation and characterization of the major protein fraction of sunflower seeds. Journal of Agricultural and Food Chemistry, 29 (3). 518-521, 19 ref..

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Abstract

The major protein fraction in sunflower seeds was isolated in a form judged homogeneous by gel filtration, gel electrophoresis and ultracentrifugation. Sunflower meal was extracted with 10% NaCl solution, and after centrifugation the supernatant was fractionated by (NH4)2SO4 precipitation; the fraction precipitating between 6.5 and 13% saturation was dissolved in 1M NaCl and dialysed against 0.025M Tris-glycine buffer pH 8.3. Sedimentation value of the isolated protein was 11.6S; mol. wt. was 300 000. The protein consisted of at least 10 major subunits as judged by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The isolated 11.6S protein contain no P, 0.31% carbohydrate, 0.31% chlorogenic acid and 2 SH groups/300 000 mol. wt. unit. 1 SH group was normally not exposed to sulphydryl reagents in the native state. Information on molecular conformation from circular dichroism studies is also presented.

Item Type:	Article
Uncontrolled Keywords:	PROTEINS-VEGETABLE; sunflower seeds, proteins analysis in; SUNFLOWERS-; proteins analysis in sunflower seeds
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 19 Lipids-oils/fats > Sunflower Seed
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	02 Mar 2018 09:14
Last Modified:	02 Mar 2018 09:14
URI:	http://ir.cftri.res.in/id/eprint/6329

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