Purification and properties of a phytohaemagglutinin from Dolichos lablab (field bean).

Rao, D. N. and Hariharan, K. and Rao, D. R. (1976) Purification and properties of a phytohaemagglutinin from Dolichos lablab (field bean). Lebensmittel Wissenschaft und Technologie, 9 (4). 246-250, 22 ref..

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Abstract

2 fractions, designated A and B, having haemagglutinin (lectin) activity were purified from aqueous extracts of field bean seeds by isoelectric precipitation, and ammonium sulphate fractionation. The haemagglutinin activity of fraction A seems to be due to presence of fraction B. Fraction B was homogeneous by acrylamide gel electrophoresis and ultracentrifugation. The mol. wt. of this fraction was approx. 100 000, as determined by gel filtration. Field bean lectin does not need metal ions for its haemagglutinating activity and among a number of sugars tested, D-glucose, D-mannose and methyl-alpha-D-mannopyranoside were inhibitory. Reversible agglutination could be demonstrated with the inhibitory sugars. Erythrocytes from rabbit, guinea pig, rat and fowl were easily agglutinated while human (A, B, O group) erythrocytes were somewhat resistant.

Item Type:	Article
Uncontrolled Keywords:	HAEMAGGLUTININS-; field bean fractions, haemagglutinins activity of; BEANS-; haemagglutinins activity of field bean fractions
Subjects:	600 Technology > 01 Medical sciences > 13 Nutrition-Human 600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Feb 2015 07:16
Last Modified:	10 Feb 2015 07:16
URI:	http://ir.cftri.res.in/id/eprint/7083

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