A method for isolation and amplification of coffee DNA using random octamer and decamer primers.

Santa Ram, A. and Sreenath, H. L. (1999) A method for isolation and amplification of coffee DNA using random octamer and decamer primers. Journal of Plantation Crops, 27 (2). 125-130 ; 9 ref..

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Abstract

Standardization of a method for the isolation of high mol. wt. DNA from the young leaves of 3 different species of Coffea L. (C. arabica L. (Rume Sudan), C. racemosa and C. congensis) is described. Optimization of components and conditions for PCR amplification using the DNA of C. arabica as template are also described. It is suggested that the standardized method may be used to generate fingerprint profiles of coffee physiologic types to show distinctions at the DNA level.

Item Type:	Article
Uncontrolled Keywords:	COFFEE-; GENETIC-TECHNIQUES; DNA-FINGERPRINTING; PCR-
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology 600 Technology > 07 Beverage Technology > 04 Coffee
Divisions:	Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Feb 2022 09:39
Last Modified:	16 Feb 2022 09:39
URI:	http://ir.cftri.res.in/id/eprint/7722

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