Effect of chemical modification of sunflower 11S protein on the binding of chlorogenic acid.

Sastry, M. C. S. and Narasinga Rao, M. S. (1991) Effect of chemical modification of sunflower 11S protein on the binding of chlorogenic acid. Journal of Agricultural and Food Chemistry, 39 (1). 63-66, 15 ref..

PDF JAFC_1991_63-.pdf Restricted to Registered users only Download (218kB)

Abstract

Binding of chlorogenic acid by sunflower 11S protein and succinylated and N-ethylmaleimide-treated protein was measured at pH 4.0 in 0.1M acetate buffer. Succinylation reduced binding, whereas N-ethylmaleimide treatment did not. Analysis of the binding data showed that succinylation reduced the number of binding sites without affecting the binding affinity. N-Ethylmaleimide treatment reduced neither the number of binding sites nor the binding affinity. Succinylation dissociated the 11S protein, whereas N-ethylmaleimide treatment did not. The secondary structure of N-ethylmaleimide-treated protein was different from that of the unmodified protein.

Item Type:	Article
Uncontrolled Keywords:	OILSEEDS-; ORGANIC-ACIDS; PHENOLS-; PROTEIN-PRODUCTS; PROTEINS-; SUNFLOWER-SEEDS; BINDING-; CHLOROGENIC-ACID; SUNFLOWERS-
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 600 Technology > 08 Food technology > 19 Lipids-oils/fats > Sunflower Seed
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Jun 2009 05:09
Last Modified:	28 Dec 2016 12:38
URI:	http://ir.cftri.res.in/id/eprint/8024

Actions (login required)

View Item