Male accessory gland secretory proteins in nasuta subgroup of Drosophila: nature and SDS-PAGE patterns.

Ram, K. R. and Ramesh, S. R. (1999) Male accessory gland secretory proteins in nasuta subgroup of Drosophila: nature and SDS-PAGE patterns. Indian Journal of Experimental Biology, 37 (8). 767-773; 39 ref..

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Abstract

Male accessory gland secretory proteins in 7 members of the D. nasuta subgroup were analyzed by SDS-PAGE in combination with different staining techniques, such as CBB-R250, Silver, PAS, PAS-silver and zinc-imidazole reverse staining. Based on coomassie blue patterns, the protein fractions could be classified in to 3 major groups, with high molecular weight fractions falling into group I and low molecular weight fractions into group III. All 3 groups of fractions are post-translationally modified by glycosylation and group III fractions are highly glycosylated. Groups I and II when localized with silver stain, and group III when localized with PAS-silver stain, appear yellow; suggesting that they are sialoglycoproteins. A 40 kD fraction of group II showed a differential staining property with zinc-imidazole stain in 2 closely-related species. Analysis of this protein fraction in F1males of an interspecific cross revealed that it is synthesized by an X-chromosomal gene.

Item Type:	Article
Uncontrolled Keywords:	accessory-glands; interspecific-hybridization; staining-; secretions-; proteins-; glycoproteins-; genes-; X-chromosome
Subjects:	600 Technology > 03 Agriculture > 05 Insect/Pest Control
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	28 Jul 2021 07:03
Last Modified:	28 Jul 2021 07:03
URI:	http://ir.cftri.res.in/id/eprint/8230

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