Isolation and characterization of immunomodulatory proteins from Tinospora cordifolia

Ramya, S. (2008) Isolation and characterization of immunomodulatory proteins from Tinospora cordifolia. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	A close look at the various immunomodulatory compounds isolated from plant sources reveals that most studies have established low molecular weight classes of compounds like alkaloids and saponins to be immunomodulators. The high molecular weight compounds studied would include polysaccharides, lignans, lipopolysaccharides and even nucleic acids. When it comes to proteins and peptide immunomodulators only a few have been studied. So an attempt was done here in this work to isolate and characterize immunomodulatory proteins from the medicinal plant Tinospora cordifolia (guduchi). Ayurveda hails guduchi as one of the medicines that can “stave off diseases”. The chemical constituents identified in this plant include alkaloids, glycosides, sterols, lactones and fatty acids. One or more of these constituents could be immunomodulatory and help in disease prevention. There have been recent reports of a 550 kD polysaccharide from guduchi possessing immunomodulatory function. The present study has focused on the protein components of guduchi; their separation, mitogenicity and preliminary characterization. Also an attempt was made to isolate, purify and test the mitogenic activity of the 550 kD polysaccharide as described by previous workers. Tinospora is rich in proteins (0.4%). The proteins in guduchi extract were separated based on the difference in their molecular weights by gel permeation chromatography. Three pools G1, G2, G3 were made accordingly and their mitogenic potency studied by the MTT assay. Cell proliferation was tested in vitro using these three pools. The G1 pool containing atleast 6 proteins, with an itensely staining protein in the 24-29 kD mol.wt. range, and G2 pool with a single purified protein 30-36kD mol. wt. range showed mitogenic activity. The presence of glycoproteins was also checked for in each of these pools by ELLSA and also confirmed by periodic acid staining. Pools G1 and G2 showed the presence of glycoproteins, (G2 pool with a single purified protein 30-36kD mol. wt. range shows intensely stained pink band in PAS is a glycoprotein) but as they were incapable of agglutinating human and rabbit erythrocytes it is confirmed that proteins have no lectin activity. Work on immune stimulating polysaccharides was done following the same procedure followed in the research paper by Raveendran Nair et al., 2004 and found out that the polysaccharide (non starchy) is mitogenic proliferating the normal rat splenocytes.
Uncontrolled Keywords:	Tinospora cordifolia immunomodulatory properties protein component polysaccharide
Subjects:	600 Technology > 03 Agriculture > 04 Medicinal Plants 600 Technology > 01 Medical sciences > 10 Immunology
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Nov 2008 05:19
Last Modified:	28 Dec 2011 10:06
URI:	http://ir.cftri.res.in/id/eprint/8834

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