Enzymatic syntheses of dopamine glycosides

Sivakumar, Ramaiah and Divakar, S. (2009) Enzymatic syntheses of dopamine glycosides. Enzyme and Microbial Technology, 44 (1). pp. 33-39. ISSN 0141-0229

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Abstract

Glucoamylase from Rhizopus mold and [beta]-glucosidase catalysed synthesis of dopamine-d-glucoside was optimized in terms of pH, buffer, enzyme, dopamine concentrations and incubation period in organic media. Under the optimum conditions, [beta]-glucosidase showed regioselectivity by giving rise to 3-hydroxy-4-O-([beta]-d-glucopyranosyl)phenylethylamine 5b. Presence of hydroxyl group at 3rd and 4th position of phenyl ring showed only monoglycosylation/arylation products with d-glucose 2, d-galactose 3 and d-mannose 4 for both the enzymes. Synthesized glycosides also exhibited ACE inhibition and antioxidant activities.

Item Type:	Article
Uncontrolled Keywords:	Enzymatic synthesis Dopamine glycosides Glucoamylase [beta]-Glucosidase ACE inhibition Antioxidant activity
Subjects:	600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering 600 Technology > 08 Food technology > 32 Antioxidants 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 Jun 2009 06:29
Last Modified:	28 Dec 2011 10:10
URI:	http://ir.cftri.res.in/id/eprint/9074

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