Possible degradation/biotransformation of lutein in vitro and in vivo: isolation and structural elucidation of lutein metabolites by HPLC and LC-MS (atmospheric pressure chemical ionization)

Rangaswamy, Lakshminarayana and Aruna, G. and Sangeetha, Ravi Kumar and Bhaskar, N. and Divakar, S. and Baskaran, V. (2008) Possible degradation/biotransformation of lutein in vitro and in vivo: isolation and structural elucidation of lutein metabolites by HPLC and LC-MS (atmospheric pressure chemical ionization). Free Radical Biology and Medicine, 45. pp. 982-993.

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Abstract

Metabolites of lutein are highly concentrated in the human macula and are known to provide protection against age-related macular degeneration. The aim of this investigation was to characterize the in vitro oxidation products of lutein obtained through photo-oxidation and to compare them with biologically transformed dietary lutein in intestine, plasma, liver, and eyes of rats. In vivo studies involved feeding rats a diet devoid of lutein for 2 weeks to induce deficiency. Rats were divided into two equal groups (n=6/group) and received either micellar lutein by gavage for 10 days or diet supplemented with fenugreek leaves as a lutein source for 4 weeks. Lutein metabolites/oxidation products obtained from in vivo and in vitro studies were characterized by HPLC and LC-MS (APCI) techniques to elucidate their structure. The characteristic fragmented ions resulting from photo-oxidation of lutein were identified as 523 (M++H+−3CH3), 476 (M++H+−6CH3), and 551 (M++H+−H2O). In the eyes, the fragmented molecules resulting from lutein were 13-Z lutein, 13′-Z lutein, 13-Z zeaxanthin, all-E zeaxanthin, 9-Z lutein, 9′-Z lutein, and 3′-oxolutein. Epoxycarotenoids were identified in liver and plasma, whereas anhydroluteinwas identified in intestine. This study emphasizes the essentiality of dietary lutein to maintain its status in the retina.

Item Type:	Article
Uncontrolled Keywords:	Free radicals Anhydrolutein Biotransformation LC-MS Lutein Lutein epoxide Metabolites Photo-oxidation
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions:	Dept. of Biochemistry Fermentation Technology and Bioengineering Meat Fish and Poultry Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Jun 2009 10:47
Last Modified:	31 May 2012 04:14
URI:	http://ir.cftri.res.in/id/eprint/9095

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