Studies on Glucose Uptake in Eat Cells by Extracts of a Potent Anti-Diabetic Plant Material

Ramya, R. (2010) Studies on Glucose Uptake in Eat Cells by Extracts of a Potent Anti-Diabetic Plant Material. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	Prevention and control of complications associated with diabetes has become one of the key issues in biomedical research. In recent years, emphasis is being placed on development of plantbased therapeutics which is basically perceived as safe. A systematic study of carbohydrate content and bioactives such as phenols on some extracts of T cordifolia and influence these extracts on glucose uptake in EAT cells was conducted. The objective was to study the glucose uptake in EAT cells by using plant extracts like T cordifolia, which is known for its antidiabetic property. Our results indicate that extracts of Tinospora cordifolia stem are rich in polyphenols, the nature of phenolic binding for polysaccharides matters a lot for bioactivity implementation. This may also indicate that number of potential phenolic binding site may be important for binding of phenolics to polysaccharide, and it also exhibit very good antioxidant properties. According to Ayurveda, T cordifolia imparts strength to tissues (balya). T cordifolia stem extracts showed rich polyphenol content, especially in ethyl acetate, methanol and ethanol extracts. The antioxidant activities were high in methanol and alcohol extracts. The HPLC profile also showed phenolic acids like catechol, vanillic acid, caffeic acid and epicatechin in different extracts of Tinospora stem, which are well-known antioxidants. GLC profile of Tinospora stem showed four major peaks among which rhamnose was identified. The other unidentified peaks may correspond to some bioactive polysaccharide in stem. Glucose uptake studies revealed that Tinospora stem extracts inhibited glucose uptake both at lower and higher dosages. The antidiabetic property of Tinospora might be through alternate mechanisms. Glucose uptake by Tinospora into cells might be mediated through some other glucose transporters other than GLUT 1 and 3, which are present in EAT cells. Further studies are required to investigate the molecular mechanisms and additional effects in glucose uptakemediated pathways for diabetes treatment.
Uncontrolled Keywords:	Diabetes mellitus; Failure,β-cells; T cordifolia stem extracts; antioxidant activities; antidiabetic property; glucose uptake
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 600 Technology > 01 Medical sciences > 04 Diabetes Mellitus
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	02 Sep 2010 09:33
Last Modified:	02 Sep 2010 09:33
URI:	http://ir.cftri.res.in/id/eprint/9633

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