Characterization of a new acid stable exo-β-1,3-glucanase of Rhizoctonia solani and its action on microbial polysaccharides

Vijayendra, S. V. N. and Yutaka, K. (2009) Characterization of a new acid stable exo-β-1,3-glucanase of Rhizoctonia solani and its action on microbial polysaccharides. International Journal of Biological Macromolecules, 44 (1). pp. 92-97.

Preview

PDF Vijayendra SVN - Kashiwagi - IJBM 2009 paper.pdf - Accepted Version Download (205kB) | Preview

Abstract

A new acid stable exo-β-1,3-glucanase of Rhizoctonia solani purified from a commercial source ‘Kitarase-M’, by a combination of ammonium sulfate precipitation, ion-exchange and gel filtration methods, had specific activity of 0.26 U/mg protein, Km and Vmax values of 0.78 mg/ml and 0.27 mM/min/mg protein, respectively. It had molecular weight of 62 kDa with optimum activity at 40 °C temperature and pH 5.0, with high stability at pH of 3–7. Unique amino acid sequence was found at N-terminal end. The substrate specificity studies confirmed that it is an exo-β-1,3-glucanase. It could hydrolyze curdlan powder to release glucose.

Item Type:	Article
Uncontrolled Keywords:	Characterization; Curdlan; Exopolysaccharides; Exo-β-1,3-glucanase; Oligosaccharides; Purification; Rhizoctonia solani
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Nov 2010 06:54
Last Modified:	27 Aug 2018 04:21
URI:	http://ir.cftri.res.in/id/eprint/9786

Actions (login required)

View Item