Neuroprotective effects of creatine against rotenone induced neurotoxicity and mitochondrial dysfunctions in Drosophila melanogaster

Ravikumar, Hosamani and Chandrashekar, K. N. and Dr., Muralidhara (2009) Neuroprotective effects of creatine against rotenone induced neurotoxicity and mitochondrial dysfunctions in Drosophila melanogaster. Journal of Neurogenetics, 23. p. 118.

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Abstract

Recent studies have shown that dietary creatine (Cr) supplementation has beneficial effects in models of acute/ chronic neurodegeneration, which are ascribed due to the bioenergetic, antiapoptotic, antiexcitotoxic and antioxidant properties. In the present study, we have attempted to obtain insights into the neuroprotective effects of Cr in a Drosophila model. The primary objective was to understand if Cr offers protection against Rotenone induced neurotoxic effects and basis of its neuroprotection. D. melanogaster (Oregon K, adults) exposed to Cr (2, 5, 10 mM, diet) for 7 days showed no mortality, while the levels of lipid peroxidation were diminished (1040%) in whole body homogenates. Further, we investigated if Cr can protect against Rotenone (ROT) induced oxidative stress, locomotor deficits and lethality. While ROT, (500 mM) caused significant mortality (95%), co-exposure with Cr offered robust protection against ROT-induced lethality and locomotor deficits (by negative geotaxis). While Cr alone had no effect on fly behavior, it markedly (50%) improved the performance of ROT flies. In a satellite study, adult flies exposed to paraquat per se (40 mM) showed significant mortality (95%, 72 h), while Cr treated flies (10 mM) exhibited higher survival rate (50%, 72 h) suggesting significant development of resistance. Our results demonstrate the potential neuroprotective effects of Cr in Drosophila, which are consistent with the hypothesis that Cr possesses significant beneficial effects against neurodegeneration.

Item Type:	Article
Uncontrolled Keywords:	dietary creatine , Drosophila, Rotenone, neurotoxic effects
Subjects:	600 Technology > 01 Medical sciences > 17 Toxicology
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Nov 2010 07:12
Last Modified:	22 Jun 2012 06:26
URI:	http://ir.cftri.res.in/id/eprint/9787

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