Influence of triacontanol on somatic embryogenesis in Coffea arabica L. and Coffea canephora P. ex Fr

Giridhar, P. and Indu, E. P. and Ravishankar, G. A. and Chandrasekar, A. (2004) Influence of triacontanol on somatic embryogenesis in Coffea arabica L. and Coffea canephora P. ex Fr. In Vitro Cellular and Developmental Biology - Plant, 40 (2). pp. 200-203.

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Abstract

A highly reproducible method for regeneration of Coffea arabica and C. canephora plants via direct somatic embryogenesis from cultured leaf and stem segments of regenerated plants was developed. Embryogenesis was influenced by the presence of triacontanol (TRIA) in the medium. TRIA incorporated at 4.55 and 11.38 mM in half-strength MS basal medium containing 1.1 mM 6-benzyladenine (BA) and 2.28mM indole-3-acetic acid (IAA) induced direct somatic embryogenesis in both species. A maximum of 260 +/- 31.8 and 59.2 +/- 12.8 somatic embryos per culture were induced from in vitro leaf explants of C. arabica and C. canephora, respectively. TRIA also induced embryo formation from in vitro stem segment callus tissues along with multiplication of primary embryos into secondary embryos. By using TRIA, it was possible to obtain somatic embryogenesis in C. arabica and C. canephora.

Item Type:	Article
Uncontrolled Keywords:	Hypocotyls; Leaf; Somatic embryos
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology 500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 07 Life Sciences > 02 Evolution & genetics
Divisions:	Plant Cell Biotechnology
Depositing User:	Users 197 not found.
Date Deposited:	01 Jun 2011 09:06
Last Modified:	28 Dec 2011 10:23
URI:	http://ir.cftri.res.in/id/eprint/10051

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