Madhusudhan, M. C. and Chethana, S. and Raghavarao, K. S. M. S. (2011) Electrokinetic Demixing of Polymer/Salt Systems Containing Biomolecules. Separation Science and Technology, 46. pp. 727-733.
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Abstract
Electrokinetic demixing is attempted for faster separation of
aqueous two-phase systems. Electrokinetic demixing is carried
out employing the PEG/potassium phosphate phase system for the
separation of biomolecules like Bovine serum albumin and
C-phycocyanin. The effect of field polarity, field strength, and
concentration of proteins on partitioning are studied at two different
tie lines. In spite of the application of the electric field, BSA as well
as C-phycocyanin partitioned to their preferred phases. Demixing
time decreased by 50% at 4.4 V/cm when compared to gravity.
Electrokinetic demixing could decrease the phase demixing time
without affecting the partitioning of the target protein.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | aqueous two phase extraction; bovine serum albumin; C-phycocyanin; demixing; electroextraction; phase separation |
| Subjects: | 600 Technology > 08 Food technology > 07 Food Engineering |
| Divisions: | Food Engineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 06 Jul 2011 06:15 |
| Last Modified: | 28 Dec 2011 10:25 |
| URI: | http://ir.cftri.res.in/id/eprint/10206 |
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