Membrane Processing for Purification and Concentration of β-glycosidases from Barley (Hordeum vulgare)

Hemavathi, A. B. and Raghavarao, K. S. M. S. (2011) Membrane Processing for Purification and Concentration of β-glycosidases from Barley (Hordeum vulgare). Biotechnology and Bioprocess Engineering, 16. 282-290 .

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Abstract

Tangential flow ultrafiltration with a polyethersulfone (100 kDa) membrane was used for the purification and concentration of β-glycosidases (β-galactosidase and β-glucosidase) from aqueous extract of barley. The performances of mode 1 (concentration followed by diafiltration) and mode 2 (diafiltration followed by concentration) were compared. In mode 1 activity recoveries of 91.44 and 88% as well as purifications of 1.84 and 1.77-fold for β-galactosidase and β-glucosidase, respectively, were obtained in a total processing time of about 9 h. In mode 2 activity recoveries of 95.68 and 91.76% with purifications of 4.56 and 4.38-fold for β-galactosidase and β-glucosidase, respectively, were obtained in a total processing time of about 6 h. The removal of total carbohydrates and protein was 56.74 and 50.73%, respectively, in mode 1, whereas it was 81.46 and 79.04%, respectively, in mode 2. The diafiltration volume and volume concentration of 3 were maintained in both mode 1 and 2. Flux decline was severe in mode 1 and led to a long processing time of about 9 h. These results indicate that mode 2 was better than mode 1 for purification of β-glycosidases.

Item Type:	Article
Uncontrolled Keywords:	β-galactosidase, β-glucosidase, concentration, diafiltration, flux, fouling
Subjects:	600 Technology > 08 Food technology > 21 Cereals > 02 Barley 600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Aug 2011 11:01
Last Modified:	19 Jun 2012 04:28
URI:	http://ir.cftri.res.in/id/eprint/10413

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