Detection and Characterization of Pediocin PA-1/AcH like Bacteriocin Producing Lactic Acid Bacteria.

Fifty-five bacteriocinogenic lactic acid bacteria
(LAB) isolated from seven different sources. Eight isolates
were found to produce pediocin PA-1 like bacteriocin as
detected by pedB gene PCR and dot-blot hybridization.
The culture filtrate (CF) activity of these isolates exhibited
strong antilisterial, antibacterial activity against tested
food-borne pathogens and LAB. The identification and
genetic diversity among the selected LAB was performed
by conventional morphological and molecular tools like
RFLP, RAPD, and 16S rDNA gene sequencing. The isolates
were identified as, 1 each of Pediococcus acidilactici
Cb1, Lactobacillus plantarum Acr2, and Streptococcus
equinus AC1, 2 were of P. pentosaceus Cb4 and R38, and
other 3 were Enterococcus faecium Acr4, BL1, V3. Partial
characterization of the bacteriocins revealed that the peptide
was heat-stable, active at acidic to alkaline pH, inactivated
by proteolytic enzymes, and had molecular weight
around 4.6 kDa and shared the properties of class IIa
pediocin-family. The bacteriocin production at different
temperatures, pH, and salt concentrations was studied to
investigate the optimal condition for application of these
isolates as a starter culture or as a biopreservative in either
acidic or non-acidic foods.