Downstream processing of luciferase from fireflies (Photinus pyralis) using aqueous two-phase extraction.

Priyanka, B. S. and Rastogi, N. K. and Raghavarao, K.S.M.S. and Thakur, M. S. (2012) Downstream processing of luciferase from fireflies (Photinus pyralis) using aqueous two-phase extraction. Process Biochemistry, 47 . pp. 1358-1363. ISSN 1359-5113

PDF Process Biochemistry, Volume 47, Issue 9, September 2012, Pages 1358-1363.pdf - Published Version Restricted to Registered users only Download (376kB)

Abstract

The firefly luciferase has been extensively used for sensitive detection of bacteria, gene expression and environmental toxins (biosensors). The aim of the present study was to design a simple and more efficient method for the purification and concentration of luciferase using aqueous two-phase extraction (ATPE). Downstream processing of luciferase from North American Firefly Photinus pyralis was carried out, for the first time, using polymer/salt aqueous two phase system (ATPS) at 4 ◦C. The enzyme was observed to preferentially partition to the polyethylene glycol (PEG) rich top phase. The best results of purification (13.69 fold) and enzyme activity recovery (118.34%) were observed in the system containing 4.0% (w/w) PEG (1500) and 20.5% (w/w) (NH4)2SO4 with a phase volume ratio of 0.21.

Item Type:	Article
Uncontrolled Keywords:	Downstream processing Luciferase, Bioseparation, Aqueous two-phase extraction Purification
Subjects:	600 Technology > 08 Food technology > 07 Food Engineering
Divisions:	Fermentation Technology and Bioengineering Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	28 Aug 2012 06:44
Last Modified:	29 Dec 2016 13:11
URI:	http://ir.cftri.res.in/id/eprint/10981

Actions (login required)

View Item