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Development of dipstick-based immunochemiluminescence techniques for the rapid detection of dichlorodiphenyltrichloroethane.
Syed, Baker and Vinayaka, A. C. and Manonmani, H. K. and Thakur, M. S. (2012) Development of dipstick-based immunochemiluminescence techniques for the rapid detection of dichlorodiphenyltrichloroethane. Luminescence, 27 (6). pp. 524-529.
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Abstract
The occurrence of organochlorine pesticides in the environment has been a major concern, due to their high persistence and the possible impacts of their exposure to humans. Dichlorodiphenyltrichloroethane (DDT) is most hazardous and one of the most widely used organochlorine insecticides. DDT and its main metabolites are highly stable to physical, chemical and biological degradation and are therefore still being detected in many parts of the world. The present study describes dipstickbased immuno-chemiluminescence method for the detection of DDT with high sensitivity. Anti-DDT antibodies raised in chicken (IgY) were used as the biological sensing elements by immobilizing onto nitrocellulosemembrane strips in a chemiluminescence (CL)-based dipstick technique. The photons generated during the biochemical interaction were directly proportional to the DDT concentration. A mean recovery of 81.2–95.6% was obtained for DDT-spiked fruit juice samples with 2.8–4.6% relative standard deviation (RSD). Using the proposed dipstick-based immuno-CL method, DDT was detected with linearity in the range 0.05–1ng/ mL, having a limit of detection (LOD) of 0.05ng/mL. This method can be used for the rapid, reliable detection of DDT pesticide.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | dipstick; DDT; chemiluminescence; IgY antibodies; immunosensor |
| Subjects: | 600 Technology > 01 Medical sciences > 17 Toxicology |
| Divisions: | Fermentation Technology and Bioengineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 01 Jan 2013 06:27 |
| Last Modified: | 08 Mar 2013 07:06 |
| URI: | http://ir.cftri.res.in/id/eprint/11076 |
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