Moringa oleifera seed protein isolate preparation: influence of hofmeister ions and optimization by rsm.

Ankit, Jain (2012) Moringa oleifera seed protein isolate preparation: influence of hofmeister ions and optimization by rsm. [Student Project Report] (Submitted)

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Abstract

Protein energy malnutrition existence necessitated extensive research into unexploited protein sources. Moringa oleifera is a highly valued vegetable plant and its seed meal contains ~52% protein. However, presence of multiple isoelectic point restricts protein isolate preparation by the conventional method. The objective of the present study is to understand influence of Hofmeister ions on M. oleifera seed protein isolate preparation and optimize protein extraction with selected salt using response surface methodology (RSM). RSM was employed to study the effect of solvent to flour ratio, salt concentration and temperature on protein extraction efficiency (PEE). Results showed that PEE with cations followed a direct Hofmeister series (Na+ ~ K+ < Mg2+ < Ca2+) while with anions followed a reverse Hofmeister series except for Cl- and Br− ions which advanced their position (SO4 2− > Cl− > Br− > HPO4 2− ~ CH3COO− > I− > SCN−). Among the ten different salts examined, Na2SO4 (69.9%), KCl (66.2%), NaCl (65.4%), and NaBr (63.5%) displayed not only better PEE but also resulted in higher PMM yield (~52% true protein) with a purity >90%. Although, anyone of these salts could be chosen, NaCl is preferred considering its wider acceptance in various applications.

Item Type:	Student Project Report
Uncontrolled Keywords:	Moringa oleifera, Response surface Methodology, Hofmeister
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	03 Apr 2014 07:32
Last Modified:	03 Apr 2014 07:32
URI:	http://ir.cftri.res.in/id/eprint/11432

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