Gobinath, D. and Prapulla, S. G. (2014) Permeabilized probiotic Lactobacillus plantarum as a source of b-galactosidase for the synthesis of prebiotic galactooligosaccharides. Biotechnology Letters, 36 (1). pp. 153-157.
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Abstract
Permeabilized probiotic Lactobacillus plantarum
was used as a source of b-galactosidase for the
synthesis of galactooligosaccharides (GOS) from lactose.
b-galactosidase activity was highest when galactose
(1,724 Miller Units) was used as a carbon source
compared to lactose, sucrose or glucose at 37 �C, 18 h.
Permeabilized cells had the highest transgalactosylation
activity resulting in 34 % (w/w) GOS synthesis from
40 % (w/v) lactose at 50 �C over 12 h. HPLC revealed
that the GOS were composed of 13 % disaccharides
(non-lactose), 17 % trisaccharides and 4 % tetrasaccharides
that were further confirmed by ESI–MS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Galactooligosaccharides � Lactobacillus plantarum Permeabilized cells Prebiotics Probiotics |
| Subjects: | 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology |
| Divisions: | Fermentation Technology and Bioengineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 12 Jun 2015 10:31 |
| Last Modified: | 29 Jun 2015 06:40 |
| URI: | http://ir.cftri.res.in/id/eprint/11781 |
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