Approaches for Biological Improvement of Eco-friendly Crops

Marker gene is a DNA sequence whose phenotype can be easily recognized and identified. It can be used
to flag another gene. A selective marker encourages the growth in the presence
of a selective agent, which
kills or prevents the growth of a cell. Antibiotics resistance gene is used to tag target gene or gene carrying
a trait of interest. Antibiotics are used in crops during transfer of gene of interest in crops improvement
programm
es. Antibiotics and antibiotics resistance gene has been used for a long time in plant
biotechnology. Not even one Gene transfer method has so much efficiency so that screening allows the
selection of transformed cells having marker gene together with targ
et gene which inactivates the antibiotic
and protect the cells from antibiotic while non transformed cells do not have marker gene so they are killed
in presence of antibiotics during screening.