Antihyperlipidemic activity of Cassia auriculata flower extract in oleic acid induced hyperlipidemia in Saccharomyces cerevisiae.

The Cassia auriculata herb has been traditionally
used in India for medicinal purposes to treat hyperglycemia,
diabetes, rheumatism, asthma, and skin diseases.
In the present study, ethanolic extract of Cassia auriculata
flower (Et-CAF) depicted anti-hyperlipidemic effect in
the budding yeast cells. The hyperlipidemic conditions
were induced in the yeast cells with oleic acid which
showed an increase in triacylglycerol (TAG) and sterol
esters (SE), and was supported by the mRNA expression of
LRO1 and DGA1 (involved in TAG formation); as well as
ARE1 and ARE2 (involved in SE formation). The antihyperlipidemic
effect by the Et-CAF was compared with
the commercial drug Atorvastatin. The lipid droplets were
increased in the hyperlipidemic yeast cell that was
observed under the confocal microscope with BODIPY
staining; Atorvastatin and Et-CAF reduced the lipid droplets.
This study revealed that the anti-hyperlipidemic
effect in Et-CAF has gained importance and might be used
to fill the gap created by the allopathic drugs.