Epigallocatechin gallate protects BEAS-2B cells from lipopolysaccharide-induced apoptosis through upregulation of gastrin-releasing peptide.

Divya, P. and Bijesh, Puthusseri and Denny Joseph, K. M. and Savanur, M. A. (2017) Epigallocatechin gallate protects BEAS-2B cells from lipopolysaccharide-induced apoptosis through upregulation of gastrin-releasing peptide. Molecular and Cellular Biochemistry, 434. pp. 105-111. ISSN 0300-8177

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Abstract

Gastrin-releasing peptide (GRP) plays a major role in the development and maintenance of lung epithelial cells by promoting cell division, whereas its suppression causes growth arrest and apoptosis. The present study shows that human bronchial epithelial BEAS-2B cells challenged with lipopolysaccharide (LPS), an endotoxin from gram-negative bacteria, downregulated GRP expression and induced apoptosis via upregulation of p53 and active caspase-3, signifying the importance of GRP in lung epithelial cell survival. However, in the presence of epigallocatechin- 3-gallate (EGCG), a polyphenol in green tea, BEAS-2B cells resisted LPS-induced apoptosis and restored the expression of GRP and its downstream effectors such as epidermal growth factor receptor and NF-jB, as analysed by immunoblotting and qPCR. Based on our findings, we objectify that cytoprotective functions of EGCG, via upregulation of GRP in cells challenged with LPS, are novel and can be further explored in a therapeutic point of view for diseases such as septic shock.

Item Type:	Article
Uncontrolled Keywords:	Gastrin-releasing peptide � Epigallocatechin gallate � Lipopolysaccharide � Bronchial epithelial cells � Apoptosis
Subjects:	600 Technology > 08 Food technology > 32 Antioxidants
Divisions:	Dept. of Biochemistry Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Dec 2017 11:37
Last Modified:	26 Dec 2017 11:37
URI:	http://ir.cftri.res.in/id/eprint/13237

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