Heterologous expression of β-carotene hydroxylase in Dunaliella salina by Agrobacterium-mediated genetic transformation.

Daris, P. Simon and Anila, N. and Gayathri, K. and Sarada, R. (2016) Heterologous expression of β-carotene hydroxylase in Dunaliella salina by Agrobacterium-mediated genetic transformation. Algal Research, 18. pp. 257-265.

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Abstract

Dunaliella salina is a halotolerant marine microalga, known for the accumulation of β-carotene. The BCH-type β- carotene hydroxylase plays a major role in the conversion of β-carotene to xanthophylls. The β-carotene hydroxylase fromthe chlorophycean microalga, Chlamydomonas reinhardtii was amplified, and the sequence alignment analysis showed that the amplified gene belongs to the group of BCH-type hydroxylases. The amplified β-carotene hydroxylase gene with rubisco promoter and chloroplast transit peptide was introduced into the genome of the D. salina by Agrobacterium-mediated genetic transformation. The carotenoid analysis showed a 3-fold increase of violaxanthin at growth stage and a 2-fold increase of zeaxanthin under stress conditions in transformed D. salina.

Item Type:	Article
Uncontrolled Keywords:	Genetic transformation Carotenoids Violaxanthin Hydroxylase Dunaliella Microalgae
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Jan 2018 05:24
Last Modified:	22 Jan 2018 05:24
URI:	http://ir.cftri.res.in/id/eprint/13355

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