In vitro multiplication of Vanilla planifolia using axillary bud explants

A clonal propagation method has been developed
for efficient multiplication of Vanilla planifolia. Multiple shoots were developed from axillary bud explants
using semi-solid Murashige and Skoog (MS) medium
supplemented with N6-benzyladenine (BA, 2 mg l–1) and
a-naphthaleneacetic acid (NAA, 1 mg l–1). The multiple
shoots were transferred to agitated liquid MS medium with
BA at 1 mg l–1 and NAA at 0.5 mg l–1 for 2–3 weeks, and
subsequently cultured on semi-solid medium. Using this
method, an average of 42 shoots were obtained from a single
axillary bud explant over a period of 134 days. Use of
an intervening liquid medium has been found to enhance
multiplication of shoots in V. planifolia.