Comparative assessment of proteins between cell lines and tissues representing carcinomas of the breast using 2-dimensional gel electrophoresis.

Venugopal, R. Bovilla and Chaitra, Rai and Mahadevaswamy, G. Kuruburu and SubbaRao, V. Madhunapantula (2020) Comparative assessment of proteins between cell lines and tissues representing carcinomas of the breast using 2-dimensional gel electrophoresis. International Journal of Research In Pharmaceutical Sciences, 11 (2). pp. 2294-2302.

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Abstract

Incidence of breast cancer in the age group of 30 to 50 is increasing at an alarming rate globally. Moreover, existing treatments are either marginally effective or resistance developed, hence development of safe and potent pharmacological agents is immediately required. However, establishment of a disease progression marker for early detection, development of safe and effective treatment agents requires identiϑication of key proteins that are exclusively expressed in advanced malignant breast tumors. 2-Dimensional gel electrophoresis (2-DGE) is one of the early methods used for the identiϑication of deregulated proteins in cancers. Therefore, the proteins of benign and malignant tumors as well as breast cancer cell lines MCF-7, SKBR-3 and MDAMB-468, were subjected to 2-DGE and relative expression calculated. Analysis of the data showed a signiϑicant increase in the intensity of 7 protein spots compared to malignant tissues and benign ones. Further, comparison of cell line proteins with tissue lysates revealed that SKBR-3 is much closer to malignant tumors, hence, may be considered for screening drug targets as well as for evaluating the efϑicacy of pharmacological agents. In conclusion, our 2- DGE identiϑied key differences and similarities in the expression of proteins between breast cancer cell lines, benign and malignant tissues.

Item Type:	Article
Uncontrolled Keywords:	2-Dimensional Gel Electrophoresis, Breast Cancer, Protein Proϑiling, Malignant Breast Cancers
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 13 Molecular Biochemistry
Divisions:	Molecular Nutrition
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 Jun 2020 06:20
Last Modified:	01 Feb 2021 06:56
URI:	http://ir.cftri.res.in/id/eprint/14387

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