Bactericidal Effects of Exiguobacterium sp GM010 Pigment Against Food-Borne Pathogens.

Ramesh Mekala, Krishna Prashanth and Saritha, G. Pandit and Mohan, A. Dhale (2020) Bactericidal Effects of Exiguobacterium sp GM010 Pigment Against Food-Borne Pathogens. Frontiers in Sustainable Food Systems.

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Abstract

Bacterium producing yellowish-orange pigment was identified (morphological, biochemical, and 16S rRNA) as Exiguobacterium sp GM010. The UV-visible spectrum of Exiguobacterium sp GM010 extract showing λ max at 465 nm revealed orange pigment characteristic. Pigment showed broad spectrum antibacterial action against gram positive and gram negative food-borne pathogens. The SYTO9 and propidium iodide (PI) staining revealed the cell membrane damage of food-borne pathogens under confocal laser scanning microscope (CLSM) indicating the bactericidal effect. This was evidenced by the fourier transform infrared (FTIR) spectrum, showing characteristic functional groups that mainly included hydroxyl, carbonyl, and carboxylic groups causing a system of delocalized electrons leading to destabilization of membrane and decrease in membrane potential that resulted in bactericidal effect. The pigment of Exiguobacterium sp GM010 were non-toxic against Artemia franciscana and can be a promising source to control the food-borne pathogens in food industries.

Item Type:	Article
Uncontrolled Keywords:	Exiguobacterium, food-borne pathogens, yellowish-orange pigment
Subjects:	600 Technology > 08 Food technology > 10 Food Microorganisms
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	31 Dec 2020 10:56
Last Modified:	31 Dec 2020 10:56
URI:	http://ir.cftri.res.in/id/eprint/14726

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