DNA aptamer selection and detection of marine biotoxin 20 Methyl Spirolide G.

Monali, Mukherjee and Srinivas, Sistla and Shivakumar, R. Veerabhadraiah and Bettadaiah, B. K. and Thakur, M. S. and Praveena, Bhatt (2021) DNA aptamer selection and detection of marine biotoxin 20 Methyl Spirolide G. Food Chemistry, 363. p. 130332. ISSN 0308-8146

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Abstract

This study reports the selection of DNA aptamer for the detection of 20 Methyl Spirolide G (SPXG). After 10 rounds of selection, the enriched pool of aptamers specific to SPXG was cloned, sequenced and clustered into seven families based on similarity. Three sequences SPX1, SPX2 and SPX7, each belonging to different clades were further evaluated for their binding affinity. Surface plasmon resonance studies determined the highest affinity KD of 0.0345x10-8 M for aptamer SPX7. A label-free microscale thermophoresis-based aptasensing using SPX7 with highest affinity, indicated a linear detection range from 1.9 to 125000 pg/mL (LOD = 0.39 pg/mL; LOQ = 1.17 pg/mL). Spiking studies in simulated contaminated samples of mussel and scallop indicated recoveries in the range of 86 to 108%. Results of this study indicate the successful development of an aptamer for detection of SPXG at picogram levels. It also opens up avenues to develop other sensing platforms for detection of SPXG using the reported aptamer.

Item Type:	Article
Uncontrolled Keywords:	20 methyl spirolide G Aptamer SELEX
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 06 Marine Microbiology
Divisions:	Fermentation Technology and Bioengineering Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	14 Jul 2021 10:01
Last Modified:	14 Jul 2021 10:01
URI:	http://ir.cftri.res.in/id/eprint/14888

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