Purifcation and characterization of a platelet aggregation inhibitor acidic phospholipase A2 from Indian saw-scaled viper (Echis carinatus ) venom

Kemparaju, K. and Krishnakantha, T. P. and Veerabasappa Gowda, T. (1999) Purifcation and characterization of a platelet aggregation inhibitor acidic phospholipase A2 from Indian saw-scaled viper (Echis carinatus ) venom. Toxicon, 37. pp. 1659-1671.

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Abstract

An acidic phospholipase A2 (EC-I-PLA2) has been puri®ed from the Indian saw-scaled viper (Echis carinatus ) venom through a combination of column chromatography and electrophoresis. EC-I-PLA2 has a molecular weight of 16000 by SDS-PAGE. It was focussed between pH 4.2 and 4.8 by isoelectro focussing. EC-I-PLA2 was non-lethal to mice and devoid of neurotoxicity, myotoxicity, anticoagulant activity and cytotoxicity. It induced mild oedema in the foot pads of mice. The puri®ed PLA2 inhibited ADP, collagen and epinephrine induced human platelet aggregation and the inhibition was both dose and time dependent.

Item Type:	Article
Uncontrolled Keywords:	Phospholipase A2 Echis carinatus venom saw-scaled viper
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	11 Oct 2007 10:49
Last Modified:	14 Dec 2016 11:34
URI:	http://ir.cftri.res.in/id/eprint/1500

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