![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Composition and structure elucidation of bovine milk glycosaminoglycans and their anti-adipogenic activity via modulation PPAR-γ and C/EBP-α.
Chaitra, Rai and Nandini, C. D. and Poornima Priyadarshini, C. G. (2021) Composition and structure elucidation of bovine milk glycosaminoglycans and their anti-adipogenic activity via modulation PPAR-γ and C/EBP-α. International Journal of Biological Macromolecules, 193. pp. 137-144. ISSN 0141-8130
![[img]](http://ir.cftri.res.in/style/images/fileicons/other.png) |
PDF
International Journal of Biological Macromolecules 193 (2021) 137–144.pdf - Published Version Restricted to Registered users only Download (4MB) | Request a copy |
Abstract
The structure function relation of Glycosaminoglycans from bovine milk (bmGAGs) has not been studied in detail. In the present study bmGAGs was isolated and structurally characterized. Chondroitin sulphate was one of the major GAGs present and had 65% of ΔDi-diSB (GlcA(2S)-GalNAc(4S)), followed by 18% of ΔDi-4S (Δ4,5HexUAα1 → 3GalNAc). Further, bmGAGs exhibited a marked anti-adipogenic effect in 3 T3-L1 cells without affecting cell viability at the concentration used. The triglyceride content treated with bmGAGs was significantly decreased as assessed by Oil-Red O staining. Peroxisome proliferator activated receptor γ (PPAR-γ) and CCAAT/enhancer-binding proteins (C/EBPα) the critical transcription factors in adipogenesis showed significant decrease in both gene and protein levels. Sterol regulatory element-binding protein 1c (SREBP-1c) that promotes the adipocyte differentiation by enhancing the activity of PPAR-γ was inversely affected by bmGAGs and the fatty acid synthase (FAS) expression was modulated. Thus, the present work is among the firsts to demonstrate an anti-adipogenic activity of bmGAGs by modulating the adipogenesis-related marker proteins and hence bmGAGs may be used as a supplement/therapeutic in the management of obesity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bovine milk glycosaminoglycans Antiadipogenic Peroxisome proliferator-activated receptorgamma (PPAR-γ) |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry |
| Divisions: | Molecular Nutrition |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 24 Nov 2021 11:04 |
| Last Modified: | 24 Nov 2021 11:04 |
| URI: | http://ir.cftri.res.in/id/eprint/15081 |
Actions (login required)
 |
View Item |