Synthesis, Characterization and Purification of a 23 Residue peptide

Rajani, K. (2007) Synthesis, Characterization and Purification of a 23 Residue peptide. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	The peptide OB-23 was synthesized using CS136 peptide synthesizer using Fmoc strategy. After synthesis the peptide was cleaved from the resin using a cleavage mixture consisted of 8.75ml TFA, 0.5mlwater, 0.5mlEDT, 0.25ml thioanisole. The peptide was precipitated using diethyl ether. The peptide was analyzed by ESI-MS and analytical RP-HPLC which showed the presence of exactly half the mass of the peptide i.e.1259.14Daltons corresponding to m/2,the mass of the peptide is 2516.85Daltons.The so analyzed peptide was purified by preparative RP-HPLC and the fractions collected for peaks were subjected to ESI-MS analysis. The fractions that showed similar mass were pooled and lyophilized. The pooled fractions from each peak were crosschecked for purity by analytical RP-HPLC. In summary a 23 residue peptide OB-23 was synthesized and purified to homogeneity.
Uncontrolled Keywords:	Peptides characterization purification
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Nov 2007 12:02
Last Modified:	28 Dec 2011 09:31
URI:	http://ir.cftri.res.in/id/eprint/1520

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