Understanding the functional attributes of foodborne pathogen, Staphylococcus aureus RecA protein in homologous recombination and antibacterial resistance.

Foodborne diseases are caused by contamination and may occur at any stage of the
food production, delivery, and consumption chain. Illness is caused by consuming
food contaminated with bacteria, viruses, parasites, or chemical substances such as
heavy metals. Foodborne diseases encompass a wide range of illnesses, from diarrhea
to cancers. Staphylococcus aureus is the most dangerous pathogenic bacteria, among
other staphylococcal bacterial strains. The recombinases present from primitive
prokaryotes to higher eukaryotes. This recombinase is crucial in DNA transactions (
replication repair and recombination). RecA, the critical regulator protein in these
processes, is highly conserved across all the organisms. To date, the role of RecA
protein in S. aureus has not been studied. We report that the recombinant purified S.
aureus RecA protein can show the canonical activities, including; DNA binding,
displacement loop formation, strand exchange reaction, ATP- hydrolysis, and
coprotease function. Further, our findings showed that the recombinant purified, anti�recombinase, S. aureus RecX protein was found to abolish S. aureus RecA
activities in vitro. A