Micropropagation and in vitro flowering in Basella alba.

Manjula, Ranganatha and Nagashree, N. Rao and Giridhar, P. and Ashwani, Sharma (2023) Micropropagation and in vitro flowering in Basella alba. Plant Cell, Tissue and Organ Culture, 154. pp. 111-119.

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Abstract

Basella alba is a perennial vine of the Basellaceae family used as a leafy vegetable, whose phytonutrients are exploited in the Indian medicinal system since antiquity for their antifungal, anticonvulsant, anti-inflammatory, antipyretic, antiulcer, and analgesic properties. Propagation of Basella through seeds has limitations for germination and flowering. In vitro regeneration was studied on MS (Murashige & Skoog) media supplemented with various plant growth regulators, indole acetic acid (IAA), Indole butyric acid (IBA), naphthalene acetic acid (NAA), N, 6-benzyl adenine (BA), kinetin (KIN), zeatin (ZEA), gibberellic acid (GA3), and adenine sulphate (ADS), silver nitrate (AgNO3) as additives. Shoot bud initiation was observed in all combinations studied, showing a good response for direct regeneration. Shoot response (84%) was observed 12 days after inoculation in 1 mg l− 1 BA + 0.1 mg l− 1 NAA. A medium containing 0.1 mg l− 1 BA + 0.5 mg l− 1 KIN + 0.1 mg l− 1 IAA was preeminent for multiple shoots (22 ± 0.13) production with average shoot length (5.81 ± 0.19) in 5 weeks (wk). Supplementation of 40 mg l− 1 AgNO3 and 40 mg l− 1 ADS to a medium containing 1 mg l− 1 BA + 0.1 mg l− 1 NAA resulted in enhanced growth of shoots, that were 5.0 ± 0.53 cm long and with 12.0 ± 0.51 leaves. In vitro flowering was observed on MS medium with 0.5 mg l− 1 BA and 0.5 mg l− 1 GA3. The survival rate of hardened plants was 90% after transferring to the soil. This protocol can be efficiently used for mass production for regeneration of genetically transformed Basella spp. and in studying its metabolite profile specially betalains.

Item Type:	Article
Uncontrolled Keywords:	Axillary bud breaking in nodal explants on culture medium with BA+KIN + IAA could induce multiple shoots and further support in vitro flowering in presence of gibberellic acid.
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Dec 2023 11:09
Last Modified:	08 Dec 2023 11:09
URI:	http://ir.cftri.res.in/id/eprint/16853

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