Enhancement of Growth and Coumarin Production in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L.cv.Lucknow local) under the Influence of Fungal Elicitors

Harsh Pal, Bais and Sudha, Govindaswamy and Ravishankar, G. A. (2000) Enhancement of Growth and Coumarin Production in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L.cv.Lucknow local) under the Influence of Fungal Elicitors. Journal of Bioscience and Bioengineering, 90 (6). pp. 648-653.

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Abstract

Studies were conducted to determine the possible production of coumarins in hairy root cultures of CWo- rium intybus L.cv.Lucknow local under the influence of microbial agents. pytlrium aphanidtwnatum and Phytopthora parasitica var. nicotzizna were c&wed and their mycelia and medium titrate were used for the elicitation of coumarin production. The media titrate of P. parasitica at 1.0% v/v added to the MS basai medium led to the maximum growth of hairy roots of C. intybus, which was 1.57-fold higher than the untreated control on the 28th day of culture, along with maximum escuUn and escuIelin yields which were 4.06- and 3.71- fold higher than the control on the 28th day. The yields of es&in and esculetjll in hairy root cultmw of chicory strongly correlated with growth. To check the effect of these fungal elicitors on endogenous polyamine metabolism, titers of totaI endogenous polyamines were analyzed. It was confirmed that the media filtrate of P. parasitica at 1.0% v/v concentration resulted in maximum accumulation of totai endogenous polyamines, wherein endogenous spermine titers were found to be maximum as compared to endogenous spermidine and putrescine titers on the 28th day. Total endogenous spermine in the a~ of 1.0% MF v/v P. paras&a was 1.3- fold higher than that of the co&o1 on the 28th day of cuiture. Maximum growth index with greater length of primary root (17.61 f 0.18 cm) and greater number of secondary and tertiary roots was recorded for the medium filtrate of P. parasitica at 1.0% v/v concentration. This study also provided an insight into the morphologicai changes in terms of branching patterns, occurring in roots under the influence of these fungal elidtors.

Item Type:	Article
Uncontrolled Keywords:	Cichorium intybus L.cv.Lucknow local, hairy root culture, polyamines, coumarins, elicitor]
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Jun 2008 06:15
Last Modified:	28 Dec 2011 09:34
URI:	http://ir.cftri.res.in/id/eprint/1774

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