Differentiating planteose and rafﬁnose using negative ion mode mass spectrometry

Lijina, P. and Gnanesh Kumar, B. S. (2023) Differentiating planteose and rafﬁnose using negative ion mode mass spectrometry. International Journal of Mass Spectrometry, 487. pp. 1-5.

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Abstract

Planteose, a non-reducing trisaccharide, is present in seeds of many higher plants. It is a structural isomer of rafﬁnose comprising a-D-galactopyranosyl linked to C (6') of fructose residue. Planteose-centric studies are at a disadvantage due to the lack of relatively more straightforward methods to identify it. In the present study, the fragmentation pattern associated with planteose was demonstrated using collision-induced dissociation in negative ion mode. MS2 of planteose revealed the presence of a unique peak at m/z 233 that is three times more intense than that at m/z 221. The m/z 221 peak is commonly seen in the MS2 of rafﬁnose. Furthermore, we evaluated the effect of the presence of a reducing end sugar building block in a similar trisaccharide (levantriose) that was revealed to affect the generation of ion at m/z 233. Overall our analysis indicated that planteose can be clearly distinguished from rafﬁnose based on fragment patterns in the negative ion mode.

Item Type:	Article
Uncontrolled Keywords:	Planteose, rafﬁnose, spectrometry, White chia seeds
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 05 Carbohydrate Biochemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Somashekar K S
Date Deposited:	06 Mar 2025 06:06
Last Modified:	06 Mar 2025 06:06
URI:	http://ir.cftri.res.in/id/eprint/19213

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