A comparative study on permeabilization treatments for in situ determination of phytase of Rhodotorula gracilis

Bindu, S. and Somashekar, D. and Joseph, R. (1998) A comparative study on permeabilization treatments for in situ determination of phytase of Rhodotorula gracilis. Letters in Applied Microbiology, 27. pp. 336-340.

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Abstract

Rhodotorula gracilis, a red yeast, was shown to produce phytase and the cultural conditions were standardized for phytase production. Permeabilization treatments using detergents, organic solvents, enzymes and physical methods were performed and compared by measuring the phytase activity of whole cells. None of the permeabilization methods except the freeze-thaw method enhanced phytase activity. The enzyme activity of a yeast suspension subjected to 15 cycles of freezing and thawing was 77·26 U g�1 cells. Phytate in feeds is an anti-nutritional factor which complexes with many minerals, rendering it unavailable to monogastric animals. Rhodotorula gracilis subjected to freeze-thawing can be used as a source of phytase in feeds to enhance phosphorus availability.

Item Type:	Article
Uncontrolled Keywords:	Rhodotorula gracilis red yeast Permeabilization treatments phytase activity
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 19 Yeast 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	02 Jul 2009 10:57
Last Modified:	28 Dec 2011 09:37
URI:	http://ir.cftri.res.in/id/eprint/1975

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