Cassia auriculata Stem Bark Ameliorates the HFD+STZ-Induced Diabetes in both in vitro and in vivo Model

Background: The secondary metabolites from natural herbs are exploited to treat various human
ailments. The present study aimed to investigate the ameliorative effect of the methanolic extract
of Cassia auriculata (C. auriculata) stem-bark in both in vitro and in vivo mice model. Materials and
Methods: In vitro standard procedures were followed to determine the phytochemical profile.
High-fat-diet+STZ was administered to 6-weeks old mice to induce type-2-diabetes. Results: In
vitro study revealed that the highest total polyphenol and flavonoid contents with anti-oxidant
and anti-diabetic property was observed in the methanolic extract of C. auriculata stem-bark.
Type-2-diabetes rats showed significantly elevated fasting blood glucose levels with decreased
serum insulin level. Further, the oxidative stress in the serum and muscle as well as altered
activities of liver enzymes of carbohydrate metabolism in diabetic control mice compared to
normal control mice was observed. The histomorphology of liver and pancreas was deleteriously
altered in diabetic group mice. However, methanolic extract of C. auriculata stem-bark (200 mg/kg;
b.wt) and metformin (200 mg/kg; b.wt) treated diabetic mice did not show the above-mentioned
alterations suggesting its ameliorative effect in type-2-diabetes. Conclusion: The methanolic
extract of C. auriculata is potent enough to be employed as antidiabetic agent.