Colorimetric determination of lactic, glutamicbutyric and choline dehydrogenase activities of rat liver using triphenyl tetrazolium bromide.

The use of triphenyltetrazolium chloride as a
redox indicator for the quantitative determination
of succinic dehydrogenase activity of liver was
first suggested by Kun and Abood 1. They
reported that the tetrazolium method possessed
the following advantages over the Thunberg
methylene blue technique~: (a) the system does
not require anaerobic conditions and (b) the
enzyme activity can be followed quantitatively
by colorimetric measurements. More recently,
Glock and Tensen3 used p-pl-diphenylenebis-z(
3, s-diphenyltetrazolium chloride) for determining
the succinic dehydrogenase activity of plant
tisSUes. In the present investigation the use of
triphenyltetrazolium bromide for determining
lactic, glutamic, butyric and choline dehydroge-
- nase activities of liver has been studied. A
preliminary account of the results are reported in
this communication.