Factors influencing direct shoot regeneration from ovary explants of saffron.

Bhagyalakshmi, N. (1999) Factors influencing direct shoot regeneration from ovary explants of saffron. Plant Cell, Tissue and Organ Culture, 58 (3). pp. 205-211.

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Abstract

Direct adventitious shoot regeneration from ovary explants of Crocus sativus L. was influenced by media components, incubation conditions, and age of the explant. The best response towards caulogenesis (28%) with highest shoot numbers per ovary was observed when full strengthMurashige and Skoog (1962) medium was supplemented with naphthaleneacetic acid and benzyladenine. Incubation in the dark at 20 �C was beneficial for induction of shoot buds. Ovaries of different growth stages having stigmas of pale yellow, pale orange and bright orange regenerated a maximummean number (3.8 – 4.2) of shoots per ovary. Further development of ovary-derived shoots was influenced by the composition of basal salts in the culture medium where full strength Murashige and Skoog salts gave the best response of those tested. Regenerated shoots produced normal photosynthetic leaves and corms.

Item Type:	Article
Uncontrolled Keywords:	Crocus sativus, geophyte, lridaceae, monocot, ovary culture, saffron, shoot regeneration
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	20 Feb 2012 06:19
Last Modified:	20 Feb 2012 06:19
URI:	http://ir.cftri.res.in/id/eprint/4185

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