Hariharan, K. and Rajagopal Rao, D. (1978) Haemagglutinins in triticales. Proceedings of the Indian Academy of Sciences, 87B. pp. 63-66.
Proceedings of the Indian Academy of Sciences, Volume-87B( (1978) 63-66.pdf - Published Version
Restricted to Registered users only
Download (122kB)
Abstract
Saline extracts of several varieties of Triticale had haemagglutinin activity
against rabbit, rat and fowl erythrocytes. In contrast to the wheat germ lectin the
Triticale lectin was inactive against human B, 0 blood group type erythrocytes and
rather high concentrations of the lectin are needed to agglutinate human A blood group
type erythrocytes. The Triticale lectin was purified about 20-fold with a 10% recovery
of activity from one of the varieties (DTS 138) by (NH,,)2S0. fractionation followed
sequentially by chromatography on DEAE-cellulose and sulphopropyl-Sephadex.
Approximately 4 1'8 of the purified lectin caused visible agglutination with trypsinised
rabbit erythrocytes. Among a variety of sugars tested D-glucose, D-mannose and
N-acetyl-D.glucosamine (2'S-7'5mM) caused inhibition of agglutination.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Triticale: haemagglutinin; animal erythrocytes; blood group specificity |
| Subjects: | 600 Technology > 08 Food technology > 16 Nutritive value 600 Technology > 08 Food technology > 21 Cereals |
| Divisions: | Dept. of Biochemistry |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 29 Dec 2016 10:30 |
| Last Modified: | 29 Dec 2016 10:30 |
| URI: | http://ir.cftri.res.in/id/eprint/4306 |
