Studies on micropropagation of Decalepis hamiltoni and Stevia rebaudiana and biotransformation of Ferulic Acid to Vanilla flavour metabolites in Decalepis hamiltoni

Shwetha, S. (2006) Studies on micropropagation of Decalepis hamiltoni and Stevia rebaudiana and biotransformation of Ferulic Acid to Vanilla flavour metabolites in Decalepis hamiltoni. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	The objective to improve the node number using TRIA was achieved and could be considered as an improvement over previous protocols. In addition to the improvement of the node number; TRIA also enhanced shoot shoot growth and chlorophyll content of leaves in D.hamiltonii.The result reveals that TRIA can be used as an effective growth regulator in the micropropagation of D.hamiltonii which is a beneficial effect for fast multiplication and conservation of this highly aromatic tuberous rooted endangered shrub. The other objective of studies on effect of reduced nitrate concentration on rooting also gave positive result wherein the protocol developed could be used for effective rooting in Decalepis hamiltonii. Production of pharmaceutically important compounds using the roots cultured under nitrate stress can be a subject of intense study in future prospect. Meanwhile good improvement on micropropagation of Stevia could also be considered as an addition to the existing protocols. Invitro rooting in Stevia without growth regulators such as IBA is a good response. Mass multiplication of Stevia could thus be achieved successfully. Using Ferulic acid as a precursor for production of Vanilla flavour metabolites using cell suspensions and normal root cultures gave a good response, as an substantial increase in the levels of these metabolites could be achieved. Normal root cultures were found to be good for production of 2H4MB and Vanillin compared to cell cultures. Biosynthesis of Vanillic acid at very high levels could be made use for large-scale production of this particular secondary metabolite for obtaining Vanillin, as Vanillic acid is cheaper compared to Ferulic acid.
Uncontrolled Keywords:	micropropagation Decalepis hamiltoni Stevia rebaudiana plant tissue culture
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	12 Apr 2007
Last Modified:	28 Dec 2011 09:26
URI:	http://ir.cftri.res.in/id/eprint/449

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