Liver and erythrocyte L-aspartate: and L-alanine: 2-oxoglutarate aminotransferases of the pyridoxine deficient rat.

The withdrawal of pyridoxine from the diet of male weading rats did not appear to affect the liver
mitochondrial L-aspartate : 2-oxoglutarate amhotransferase, but the ismnzymes in the liver supematant
and in the erythrocyte showed gradual lowering of activity. The liver kalanine : Zoxoglutarate
aminatransferase activity (mostly residing in the supernatant) showed a sharp and immediate decline,
and that of the erythrocyte exhibited a more gradual decline with the progress of deficiency.
The liver supernatant and erythrocyte aspartate aminotransferases of the deficient animals showed a
marked recowry with subcutaneous injection of pyridoxine and in vitro addition of pyridoxal-5-phosphate
to the enzyme system. The data emphasize the sensitivity of the supernatant and erythrocyte aspartate
aminotransfemses in reflecting the pyridoxine nutritional state of the rat.